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PRODID:-//Cancer Epigenetics Society - ECPv5.3.2.1//NONSGML v1.0//EN
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X-WR-CALNAME:Cancer Epigenetics Society
X-ORIGINAL-URL:https://ces.b2sg.org
X-WR-CALDESC:Events for Cancer Epigenetics Society
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DTSTART:20190331T010000
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DTSTART:20191027T010000
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DTSTART;VALUE=DATE:20190521
DTEND;VALUE=DATE:20190623
DTSTAMP:20260729T153501
CREATED:20190521T120620Z
LAST-MODIFIED:20190521T123349Z
UID:21253-1558396800-1561247999@ces.b2sg.org
SUMMARY:Senior Research Associate
DESCRIPTION:In collaboration with the Principal Investigator\, under general supervision\, the Senior Research Associate will be responsible for making independent contributions to research and experiments. Incumbent will perform a) isolation of four cell types (hepatocytes\, Hepatic Stellate Cells\, Kupffer cells\, and Endothelial cells) from livers which are rejected for transplantation; b) cell characterization and cryopreservation\, storage\, cataloging; c) in vitro studies using cell stimulation\, co-culturing; d) generation of humanized mice. Incumbent will develop an independent scientific project focused on NASH and ASH-induced cross-talk between human hepatocytes/HSCs/Kupffer cells\, characterization of their gene expression profile and epigenetic signature. Will assist in writing grant applications\, applying for NIH funds to support the Human Cell Isolation lab and research projects. Will contribute to the preparation and editing of grant review proposals for federal\, state and private funding agencies. Will draft\, edit or criticize proposal aims and research plans. \nThe Senior Research Associate will manage and coordinate research efforts with other collaborators as appropriate for multidisciplinary research. Plan\, execute and follow-up on high level regular and ad hoc meetings of investigators and management. Independently conceive\, plan\, and conduct research projects based on reviews of scientific literature and recent advances in related fields\, organizing and executing all techniques involved. Contribute novel ideas of major methodological significance to the research projects\, designing key experiments and developing new techniques. Will perform complex data analysis using various statistical tools. Will author or co-author scientific journal manuscripts and book chapters and report directly to funding agencies and journal editors. Prepare and implement research study budgets based on analysis of data. Perform other duties as needed. \nThe Senior Research Associate will contribute significant\, innovative and original ideas and recommendations concerning challenging experimental procedures\, using specialized knowledge and a high level of technical skill and competence. The Senior Research Associate will develop and adapt new techniques and modify established protocols to further experiments. Interpretation of data and results of experiments for report production\, while also recognizing and investigating misleading results. \nMINIMUM QUALIFICATIONS\n\nBachelor’s degree in biology\, molecular biology\, biochemistry\, or related field and senior level laboratory experience.\nExperience with hepatocytes\, hepatic stellate\, kupffer and endothelial cells\, biochemistry\, cellular and molecular biology\, including experience in establishing\, maintaining\, transfecting\, and selecting clones from primary liver cell preparations with expertise in sterile technique.\nExperience with characterizing and behavior of human liver cell types\, specifically hepatocytes\, hepatic stellate cells\, kupffer cells and endothelial cells.\nProven experience with primary cell culture\, including large scale cell culture. Strong proven experience performing cell culture techniques including demonstrated ability working with sterile equipment and supplies. Ability to troubleshoot problems during all aspects of cell isolation and culture.\nExperience in managing multiple projects and working under milestones driven projects\, including knowledge in writing reports and experience in communicating with funding agencies.\nExperience in technical direction and training of students\, post-docs\, and technicians.\nDemonstrated experience in DNA and RNA extraction and use of extracted products in various PCR methods.\nExperience in designing and performing next generation RNA sequencing samples preparation and analyses.\nExceptional experience and knowledge in developing hypotheses\, design experiments\, conduct experiments\, analyze data\, write manuscripts\, and publish results in peer-reviewed journals\, including all interactions with Reviewers and Editors.\nDemonstrated experience in mouse models of liver fibrosis and cancer.\nExperience performing surgical implantation of shRNA-transfected tumor cell lines and intra-hepatic injections of human primary cells.\nProven experience with primary cell isolation from whole liver\, including large scale isolations.\nDemonstrated experience with standard biochemical and molecular biological laboratory procedures such as DNA cloning\, gel electrophoresis (including knowledge of SDS-PAGE and Coomassie Blue Staining)\, PCR\, RNA purification\, reverse transcription\, real time quantitative PCR\, Western Blotting\, ELISA and Histological analysis including paraffin and frozen block making\, sectioning and staining.\nProven experience with qPCR analysis\, and immunohistochemistry.\n\nPREFERRED QUALIFICATIONS\n\nProven experience in successful preparation of research grant proposals for funding by NIH or other major funding source.\nMaster’s degree or Doctoral degree in a related field.\n\nStrong organizational skills\, including experience in laboratory record keeping\, budget analysis and managing logistics.
URL:https://ces.b2sg.org/event/21may19/
LOCATION:UC San Diego Health – Moores Cancer Center\, 3855 Health Sciences Drive\, La Jolla\, CA\, 92037\, United States
CATEGORIES:Jobs
ATTACH;FMTTYPE=image/jpeg:https://ces.b2sg.org/wp-content/uploads/2017/05/UCSDMCC.jpg
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