BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Cancer Epigenetics Society - ECPv5.3.2.1//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Cancer Epigenetics Society
X-ORIGINAL-URL:https://ces.b2sg.org
X-WR-CALDESC:Events for Cancer Epigenetics Society
BEGIN:VTIMEZONE
TZID:Europe/Paris
BEGIN:DAYLIGHT
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
TZNAME:CEST
DTSTART:20190331T010000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
TZNAME:CET
DTSTART:20191027T010000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;VALUE=DATE:20190322
DTEND;VALUE=DATE:20190424
DTSTAMP:20260729T211141
CREATED:20190322T212029Z
LAST-MODIFIED:20190322T212029Z
UID:21128-1553212800-1556063999@ces.b2sg.org
SUMMARY:Research Scientist Engineer 1
DESCRIPTION:The Dog Aging Project at the University of Washington currently has an outstanding opportunity for a RESEARCH SCIENTIST ENGINEER 1 to assist with our exploration of the epigenetic basis for aging in companion dogs.  \nOur goal is to understand how chromatin accessibility changes in dogs during the aging process\, to define epigenetic loci that are altered during the aging process\, and to establish an “epigenetic clock” for aging in companion dogs. To this end\, we seek a member of our team to refine and implement a pipeline for collection of PBMCs from donated blood samples\, quality control and flow cytometric analysis and sorting of these samples\, isolation and transposition of chromatin\, and preparation of ATAC-Seq libraries.  \nWe are seeking someone with a strong background in biology\, strong lab skills in molecular and cell biology\, and experience with chromatin biology\, next-generation sequencing library preparation\, and flow cytometry. Strong organizational skills are a must\, as this project comprises potentially hundreds to thousands of samples.  \nResponsibilities:  \nThe Research Scientist/Engineer 1 will:  \n\nIsolate PBMCs from donated blood samples. \nPerform quality control analysis on white blood cells\, including cell density and viability assessments. \nPerform flow cytometric analysis and possible sorting. \nPerform chromatin transposition (ATAC) on thawed whole or sorted cell samples. \nPrepare ATAC-SEQ libraries for next-gen sequencing analysis. \nEstablish a robust and standardized pipeline for the above steps\, including an efficient and well-organized workflow for numerous samples. \nContribute to regular meetings and one-on-one discussions about project design and progress. \nMinimum Requirements: \nBachelor’s degree in cell and molecular biology or a related discipline and at least one year of research experience of cell biology in a lab setting. \nEquivalent education/experience will substitute for all minimum qualifications except when there are legal requirements\, such as a license/certification/registration.  \nOther required qualifications include: \nPractical understanding of the principles of epigenetics and genetic regulation \nVery strong organizational skills. \nAbility to learn and integrate new analysis methods. \nExcellent oral and written communication skills. \nAbility and enthusiasm for working independently\, and also collaboratively as part of a team \nDesired: \nFlow cytometry experience \nNext-gen sequencing library preparation experience on the Illumina platform. \nPractical understanding of the cell biology of aging. \nBroad experience with next-generation sequence analysis\, including bwa\, bowtie/tophat/cufflinks\, DeSeq\, MACS2\, Galaxy\, HOMER\, or other standard next-gen software analysis software.
URL:https://ces.b2sg.org/event/22mar19/
LOCATION:University of Washington\, Seattle\, WA\, United States
CATEGORIES:Jobs
ATTACH;FMTTYPE=image/png:https://ces.b2sg.org/wp-content/uploads/2018/12/UW_uni-washington-uw.png
END:VEVENT
END:VCALENDAR