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PRODID:-//Cancer Epigenetics Society - ECPv5.3.2.1//NONSGML v1.0//EN
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METHOD:PUBLISH
X-WR-CALNAME:Cancer Epigenetics Society
X-ORIGINAL-URL:https://ces.b2sg.org
X-WR-CALDESC:Events for Cancer Epigenetics Society
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TZID:Europe/Paris
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DTSTART:20170326T010000
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DTSTART:20171029T010000
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BEGIN:VEVENT
DTSTART;VALUE=DATE:20170511
DTEND;VALUE=DATE:20170525
DTSTAMP:20260729T195316
CREATED:20170511T073353Z
LAST-MODIFIED:20170511T074400Z
UID:17963-1494460800-1495670399@ces.b2sg.org
SUMMARY:Research Associate
DESCRIPTION:Under supervision of a Principal Investigator\, incumbent will assist with the application of cellular and molecular biology techniques to research efforts related to elucidating fundamental mechanisms involved in cellular signaling pathways for the use in the development of novel targeted therapeutic agents. Assist in the investigation of intracellular signaling and epigenetic events and their respective roles in processes associated with normal cell growth and tumor progression. Perform routine laboratory experiments\, such as DNA/RNA/protein isolation\, PCR and real-time PCR\, protein and chromatin immunoprecipitation\, immunoblotting\, immunofluorescence and confocal microscopy\, and cell viability and apoptosis assays from in vitro cell lines and mouse and human tumor samples. Under supervision conduct DNA preparation\, protein expression\, and purification and assist in analysis of recombinant proteins produced in prokaryotic and eukaryotic systems. Assist in performing animal work with in vivo experiments\, including mating\, genotyping\, tumor xenografting\, and pharmacologic treatment. Collect and dissociate human tumor specimens for cell culture\, patient-derived xenografting\, and genomic\, transcriptomic/epigenomic analyses. Prepare common laboratory solutions and reagents. Assist with inventory of laboratory reagents and supplies. \nMinimum Qualifications: \n\nTheoretical knowledge of cellular\, molecular and genetic biology.\nKnowledge of cell/tissue culture such as performing recombinant engineering and/or selection/isolation of transient and/or stable transgene-expressing cells for phenotypic analyses in downstream studies\, including the use of viral vectors for gene delivery.\nAbility to perform DNA/RNA isolation\, assays\, ELISA\, PCR\, vector purifications\, tissue lysates\, immunoblotting\, and immunostaining of isolated cells and fixed/frozen tissues.\nKnowledge of cellular and molecular biology techniques\, genetic studies\, and PCR analysis. Ability to learn and carry out immunological procedures\, such as tissue lysates\, immunoblotting\, and immunostaining of isolated cells and fixed/frozen tissues.\nWillingness to handle and work with small animals.\nKnowledge of animal handling procedures\, especially mice and preferably\, nude mice and NSG. Knowledge of performing genotyping.\nFamiliarity with aseptic and sterile techniques.\nStrong communications skills (both written and verbal) needed to interact professionally and effectively in the work environment. Demonstrated ability to read\, comprehend and discuss research materials and protocols.\n\nPreferred Qualifications \n\nTheoretical knowledge of cancer research (preferable to have experience with tumor microenvironment\, cancer stem cells\, and/or drug screening).\nExperience with animal handling\, breeding\, euthanasia\, and tissue recovery. Familiarity with UCSD IACUC policies.\n\nSpecial Conditions: \n\nMust be willing to work with small animals.\nMust be willing and able to occasionally work on evenings and weekends.\n\nMust be willing and able to work with hazardous materials.
URL:https://ces.b2sg.org/event/research-associate/
LOCATION:UC San Diego Health – Moores Cancer Center\, 3855 Health Sciences Drive\, La Jolla\, CA\, 92037\, United States
CATEGORIES:Jobs
ATTACH;FMTTYPE=image/jpeg:https://ces.b2sg.org/wp-content/uploads/2017/05/UCSDMCC.jpg
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