Pfizer is seeking applications for an exceptional non-PhD scientist with chemical biology, cell biology and mass spectrometry-based proteomics experience. Such a candidate will be a highly motivated and creative scientist with the desire to join and support chemical biology and proteomics capabilities for our oncology pipeline. This person will align across Pfizer’s tumor cell biology groups enabling impact across the small molecule oncology portfolio from early exploratory target identification through the drug discovery pipeline and into early clinical development. 

The individual will support and aid in the development and application of chemical biology and innovative mass spectrometry platforms. Their work will drive a deeper understanding of our molecules and cellular targets. Relevant methodologies include but are not limited to: identification of novel oncology targets, small molecule target deconvolution, small molecule target occupancy studies using chemical probes, direct substrate identification and characterization, protein turnover studies, protein post-translation modification studies and small molecule–protein interactome analyses.

ROLE RESPONSIBILITIES

• Apply quantitative protein mass spectrometry technologies for chemo-proteomics and general proteomics across the oncology portfolio.
• Participate in all aspects of laboratory operations including sample preparation, running samples on the MS, data analysis and interpretation.
• Development of methods for direct identification of kinase substrates.
• Presenting and reporting data with follow up discussions for project teams including data impact and interpretation.
• Complete and effectively manage multiple projects within timelines ensuring that all tasks and responsibilities are carried out according to high scientific and ethical standards.
• Engage in collaborative and strategic partnerships with internal research teams to apply proteomics for oncology discovery, impacting all oncology research focuses including: Systems Therapeutics, Tumor Intrinsic Biology, Immuno-oncology and Epigenetics.

BASIC QUALIFICATIONS

• A qualified applicant holds a M.S/B.S. in biological sciences, chemical biology, analytical chemistry, or a related field.
• 6+ years of research experience with applied cell biology, chemical biology and mass spectrometry-based proteomics desired.
• Expertise in growing, maintaining and harvesting cancer cells lines.
• General understanding of chemical proteomics methodologies including small-molecule affinity enrichment, target occupancy and Chem-Seq. 
• General understanding of target deconvolution methods including CETSA, DARTS, FUSION and chemical probes/proteomics.
• Excellent verbal and written communication skills.
• Knowledge and application of complex sample preparation methodologies as applied to protein mass spectrometry workflows.
• Ability and understanding of methods to characterize protein post-translational modifications including phosphorylation, methylation, acetylation, PARylation and ubiquitination.
• Experience and knowledge in the operation of different types of mass spectrometers including Q Exactive Hybrid Quadrupole and Orbitrap Fusion Lumos Tribrid.