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Research Laboratory Technician
May 30, 2018 - July 1, 2018
A Research Laboratory Technician position is available to work on brain tumors research. Working independently under the direction of the Principal Investigator Dr. Mario Suvà in the research laboratory within the Department of Pathology, MGH, the Research Technician II is required to develop and successfully implement various new technologies and coordinate computational data analysis.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
The principle responsibility of the technician is to carry out the technical aspects of molecular testing and cancer epigenetics in gliomas, with the guidance of the PI. The specific responsibilities include:
· Performing technical components of molecular assays, including, but not limited to, the development, testing, and analysis of next-generation sequencing. The laboratory is developing two technologies applied directly to patient tumors: single cell RNA- sequencing and epigenomic profiling by chromatin-immunoprecipitation
· Derivation of short-term primary cultures from patient tumors
· Perform functional genes knock-out using novel genome-editing technologies such as the CRISPR/cas9 system. Perform in vitro drug treatment of primary cancer cell lines, and analysis of cell responses using immunohistochemistry and western blotting
· Ensuring specimen integrity
· Routine lab tasks including glassware cleaning and equipment maintenance and QC programs (on a shared schedule)
· Ensuring patient privacy and compliance with HIPAA guidelines
· Maintaining and updating the Research laboratory manual in accordance with the regulations
· Education of residents, fellows, medical students and technicians
· Work in a collaborative fashion with other group members
· Manages orders for the lab
SKILLS/ABILITIES/COMPETENCIES REQUIRED :
- Excellent communication skills, both oral and written.
- Ability to identify problems and develop solutions.
- Familiarity with molecular biology, computers and the metric system.
- Knowledge of next-generation sequencing.
- Familiarity and understanding of the concepts of molarity, normality, molecular weight, formula weight of chemicals.
- Must be familiar with how to make dilutions from stock solutions.
- Knowledge of good laboratory techniques and the scientific method.
- Ability to do mathematical calculations.
- Must know the proper handling procedure of chemicals and sterile tissue culture technique.
- Ability to prioritize, work within deadlines and multi-task.
- High degree of computer literacy.
Qualifications
EDUCATION:
BA/BS degree in Biological or Physical Sciences required.
EXPERIENCE :
A minimum of 1-2yrs of directly related and progressively more responsible experience.
Previous experience in epigenomic profiling and RNA-sequencing is required.