Welcome to the Cancer Epigenetics Cell Line Database (CECD)! Here you will find an ID card summarizing some of the known properties for each cell line reported in the Cancer Epigenetics Drug Database (CEDD). One may also access this page by clicking on the name of the cell line in the Cancer Epigenetics Drug Database.

This page is periodically updated, so please check it again for new data that may be relevant to your research. You are welcome to contribute to this database and may do so by emailing us the data and source of information at info@ces.b2sg.org, Subject line: CECD.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

22Rv1

General Information
Cancer	Human prostate carcinoma
Gender	Male
Tissue	Prostate
Cell characteristics	Epithelial, adherent
Tumorigenic in nude mice	Yes
Genetic background	Carries more than 10 genome-integrated copies of the xenotropic murine leukemia virus-related virus
p53 status	Q331R
Karyotype	49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+1,+3,+7,+8,+12
Antigen expression	Prostate-specific antigen (PSA)
STR Profile	Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,12 D16S539: 12 D5S818: 11,12,13 D7S820: 9,10,11 THO1: 6,9.3 TPOX: 8 vWA: 15,21
Virus	EBV^–, HBV^–, HCV^–, HIV^–, HTLVI^–, HTLVII^–, SMRV^–, XMRV⁺
Parental cell line	CWR22R
Doubling Time	40 hours
Culture Medium	RPMI-1640
Additional Information	Active viruses are secreted into the cell culture supernatant
Experimental Data
EpiDrugs	Hydralazine

6-10B

General Information
Cancer	Human nasopharyngeal cancer
Cell characteristics	Low tumorigenicity, non-metastatic
Virus	EBV⁺
Parental cell line	SUNE-1
Culture Medium	RPMI-1640 + 10% FBS
Experimental Data
EpiDrugs	5-aza-2′-deoxycytidine

A

A2780

General Information
Cancer	Human ovarian carcinoma
Gender	Female
Tissue	Ovary
Cell characteristics	Epithelial, adherent
p53 status	V172F
Other mutations	ARID1A, BRAF, PIK3CA, PTEN
STR Profile	Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16
Culture Medium	RPMI-1640 + 2mM glutamine + 10% FBS
Additional information	Established from an untreated patient
Experimental Data
EpiDrugs	SGI-110

A4573

General Information
Cancer	Human Ewing’s sarcoma
Gender	Female
Experimental Data
EpiDrugs	5-aza-2′-deoxycytidine

A549

General Information
Cancer	Human lung carcinoma
Gender	Male, causasian
Age	58 years
Tissue	Lung
Cell characteristics	Adherent, epithelial
Antigen expression	Positive for keratin by immunoperoxidase staining
STR Profile	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14
Doubling Time	About 22 hours
Culture Medium	Ham′s F12K or DMEM + 2mM glutamine + 10% FBS
Additional Information	Synthesizes lecithin
Experimental Data
EpiDrugs	5-aza-2′-deoxycytidine

A673

General Information
Cancer	Human Ewing’s sarcoma
Gender	Female
Age	15
Tissue	Muscle
Cell characteristics	Adherent
Tumorigenic in nude mice	Yes
STR Profile	Amelogenin: X CSF1PO: 11,12 D13S317: 8,13 D16S539: 11 D5S818: 11,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 15,18
Culture medium	DMEM + 10% FBS
Experimental Data
EpiDrugs	5-aza-2′-deoxycytidine

C

CaSki

General Information
Cancer	Human epidermoid carcinoma
Gender	Female
Age	40 years
Tissue	Cervix; derived from a metastatic site in the small intestine
Cell characteristics	Epithelial, adherent
Genetic background	PIK3CA (heterozygous for E545K)
Antigen expression	Tumor-associated antigen; human chorionic gonadotropin hCGβ
STR Profile	Amelogenin: X CSF1PO: 10 D13S317: 8,12 D16S539: 11,12 D5S818: 13 D7S820: 8,11 THO1: 7 TPOX: 8 vWA: 17
Virus	HPV-16 (~600 copies/cell)
Experimental Data
EpiDrugs	Hydralazine

CNE-1

General Information
Cancer	Human nasopharyngeal cancer
Experimental Data
EpiDrugs	5-aza-2’-deoxycytidine

D

DU-145

General Information
Cancer	Human prostate carcinoma
Gender	Male
Age	69 years
Tissue	Prostate; derived from a metastatic site: brain
Cell characteristics	Epithelial, adherent
Tumorigenic in nude mice	Yes, forms adenocarcinoma, grade II
Genetic background	Hypotriploid (61 and 62 chromosome counts)
Karyotype
Antigen expression	Blood Type O; Rh⁺
STR Profile	Amelogenin: X, Y D7S820: 7, 10, 11 D13S317: 12, 13, 14 D5S818: 10, 13 vWA: 17, 18, 19 THO1: 7 CSF1PO: 10, 11 TPOX: 11 D16S539: 11, 13
Additional information	Does not appear to be hormone-sensitive, is only weakly acid phosphatase positive, does not express prostate-specific antigen (PSA). Isolated cells form colonies in soft agar.
Experimental Data
EpiDrugs	Genistein, Hydralazine

H

H1975

General Information
Cancer	Non-small cell lung cancer, adenocarcinoma
Gender	Female
Tissue	Lung
Cell characteristics	Epithelial, adherent
Genetic background	Tumor is from a non-smoker
STR Profile	Amelogenin: X CSF1PO: 12 D13S317: 10,13 D16S539: 9,12 D5S818: 11,12 D7S820: 8,11 THO1: 7 TPOX: 8,11 vWA: 18
Experimental Data
EpiDrugs	5-aza-2’-deoxycytidine

HCT116

General Information
Cancer	Colorectal carcinoma
Gender	Male
Age bracket	Adult
Tissue	Colon
Cell characteristics	Epithelial, adherent
Tumorigenic in nude mice	Yes
Genetic background	Mutant Ras (codon 13)
Antigen expression	Carcinoembryonic antigen (CEA)
STR Profile	Amelogenin: X,Y CSF1PO: 7,10 D13S317: 10,12 D16S539: 11,13 D5S818: 10,11 D7S820: 11,12 THO1: 8,9 TPOX: 8,9 vWA: 17,22
Experimental Data
EpiDrugs	5-azacytidine

HeLa

General Information
Cancer	Cervical carcinoma
Gender	Female
Age	31
Tissue	Cervix
Cell characteristics	Epithelial, adherent
Genetic background	Low p53 expression, normal pRB levels
STR Profile	Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18
Virus	EBV^–, HBV^–, HCV^–, HHV-8^–, HIV^–, HPV-18⁺, HTLV-I/II^–, MLV^–, SMRV^–
Doubling Time	48h
Experimental Data
EpiDrugs	5-azacytidine, Hydralazine

Hep3B

General Information
Cancer	Hepatocellular carcinoma
Gender	Male
Age	8 years
Tissue	Liver
Cell characteristics	Epithelial, adherent
Tumorigenic in nude mice	Yes
Genetic background	Contains a 2.3 kb integrated hepatitis B virus genome fragment
Antigen expression	Hepatitis B surface antigen (HBsAg)
STR Profile	Amelogenin: X CSF1PO: 8 D13S317: 12,14 D16S539: 10 D5S818: 13 D7S820: 8,10 THO1: 6,7 TPOX: 9 vWA: 17
Virus	EBV^–, HBV⁺, HCV^–, HIV^–, HTLV-I/II^–, MLV^–, SMRV^–
Doubling Time	40-50 hours
Culture Medium	90% MEM (with Earle’s salts) + 10% heat-inactivated FBS + 2 mM L-glutamine
Additional information	Produces α-fetoprotein, albumin, transferrin, α2-macroglobulin, α1-antitrypsin, and haptoglobin
Experimental Data
EpiDrugs	SGI-110

Hep2G

General Information
Cancer	cervix carcinoma
Gender	female
Age	31
Tissue	cervix
Cell characteristics	epithelial, adherent
Genetic background	HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
STR Profile	Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18
Virus	EBV -, HBV -, HCV -, HHV-8 -, HIV -, HPV +, HTLV-I/II -, MLV -, SMRV –
Doubling Time	48h
Experimental Data
EpiDrugs	5-azacytidine Hydralazine

HLE

General Information
Cancer	hepatoma (non-differentiated)
Gender	68 years
Age	male
Tissue	liver, gallbladder
Virus	CMV-, EBV-, HHV6-, HHV7-, BKV-, JCV-, ADV-, parvoB19-, HBV-, HTLV1-, HTLV2-, HIV1-, HIV2-, HPV18-
Culture Medium	Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum
Experimental Data
EpiDrugs	5-azacytidine

HLF

General Information
Cancer	cervix carcinoma
Gender	female
Age	31
Tissue	cervix
Cell characteristics	epithelial, adherent
Genetic background	HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
STR Profile	Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18
Virus	EBV -, HBV -, HCV -, HHV-8 -, HIV -, HPV +, HTLV-I/II -, MLV -, SMRV –
Doubling Time	48h
Experimental Data
EpiDrugs	5-azacytidine Hydralazine

Huh7

General Information
Cancer	hepato cellular carcinoma
Gender	male
Age	57 years
Tissue	liver, gallbladder
Cell characteristics	epithelial
Genetic background	well differenciated hepatoma, infinite lifespan
Virus	CMV-, EBV-, HHV6-, HHV7-,BKJ-, JCV-, ADV1-, parvoB19-, HBV-, HTLV1-, HTLV2-, HIV1-, HIV2-
Culture Medium	Dulbecco’s minimal essential medium with 10% fetal bovine serum
Experimental Data
EpiDrugs	5-azacytidine

K

KG-1

General Information
Cancer	erythroleukemia
Gender	male
Age	59 years
Tissue	bone marrow
Cell characteristics	myeloblast-like
Genetic background	KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.
STR Profile	Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19
Culture Medium	IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs	5-aza-2′-deoxycytidine

KYSE150

General Information
Cancer	esophageal squamous cell carcinoma
Gender	female
Age	49 years
Tissue	upper (cervical) esophagus
Cell characteristics	epithelial, adherent
Tumorigenic in nude mice	yes
Genetic background	Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures)
Virus	EBV -, HBV -, HCV -, HHV-8 -, HIV -, HPV -, HTLV-I/II -, MLV -, SMRV –
Doubling Time	20-25 h
Experimental Data
EpiDrugs	5-fluoro-2′-deoxycytidine

L

LNCaP

General Information
Cancer	prostate carcinoma
Gender	male
Age	50 years
Tissue	metastasis (left supraclavicular lymph node ), prostate
Cell characteristics	epithelial, adherent (single cells and loosley attached clusters)
Tumorigenic in nude mice	Yes
Genetic background
Antigen expression	prostate specific antigen
STR Profile	Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11 D5S818: 11,12 D7S820: 9.1,10.3 THO1: 9 TPOX: 8,9 vWA: 16,18
Doubling Time	34h
Culture Medium	80% RPMI 1640 + 20% h.i. FBS
Experimental Data
EpiDrugs	Hydralazine

M

MCF7

General Information
Cancer	breast adenocarcinoma
Gender	female
Age	69 years
Tissue	metastatic site (mammary gland), pleural effusion
Cell characteristics	epithelial, adherent
Genetic background	The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.
Karyotype
Antigen expression	Blood Type O; Rh+cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin –
STR Profile	Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11,12 D5S818: 11,12 D7S820: 8,9 THO1: 6 TPOX: 9,12 vWA: 14,15
Viruses	EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV –
	50h
Culture Medium	90% RPMI 1640 + 10% h.i. FBS + MEM non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin
Experimental Data
EpiDrugs	5-fluoro-2′-deoxycytidine Genistein

MDA-MB-231

General Information
Cancer	breast cancer
Gender	female
Age	51 years
Tissue	metastatic site (mammary gland/breast; pleural effusion)
Cell characteristics	epitherlial, adherent
Tumorigenic in nude mice	Yes (forms poorly differentiated adenocarcinoma)
Genetic background	Modal no.′s 62 and 64, near triploid
Karyotype
STR Profile	Amelogenin: X CSF1PO: 12,13 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 8,9 THO1: 7,9.3 TPOX: 8,9 vWA: 15,18
Culture Medium	L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS
Experimental Data
EpiDrugs	Genistein

MRC-5

General Information
Cancer	non
Gender	male
Age	14 weeks gestation
Tissue	lung
Cell characteristics	fibroblast, adherent
Tumorigenic in nude mice	no
Genetic background	he cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38
STR Profile	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 9,11 D5S818: 11,12 D7S820: 10,11 THO1: 8 TPOX: 8 vWA: 15
Culture Medium	EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs	Genistein

P

PC3

General Information
Cancer	prostate adenocarcinoma, grade IV
Gender	male
Age	62 years
Tissue	metastatic site (bone)
Cell characteristics	epithelial
Tumorigenic in nude mice	yes
Genetic background	The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
Antigen expression	HLA A1, A9
STR Profile	Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,11 THO1: 6,7 TPOX: 8,9 vWA: 17
Culture Medium	EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs	Hydralazine Genistein

R

RPMI8226

General Information
Cancer	plasmacytoma, myeloma
Gender	male
Age	61 years
Tissue	peripheral blood
Cell characteristics	lymphoblast, suspension
Genetic background	There is no evidence of heavy chain production (cytoplasmic or secreted).
Karyotype	Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres.
Antigen expression	HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
STR Profile	Amelogenin: X,Y CSF1PO: 12 D13S317: 11 D16S539: 9 D5S818: 11,13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16,18
Culture Medium	RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs	5-azacytidine

S

SiHa

General Information
Cancer	squamous cell carcinoma (grade II)
Gender	female
Age	55 years
Tissue	cervix
Cell characteristics	epithelial, adherent
Tumorigenic in nude mice	Yes
Genetic background	Oncogenes: p53 +; pRB +
STR Profile	Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12 D5S818: 9 D7S820: 10 THO1: 6,9 TPOX: 8 vWA: 14,17
Experimental Data
EpiDrugs	5-azacytidine; Hydralazine

SKOV3

General Information
Cancer	adenocarcinoma
Gender	female
Age	64 years
Tissue	ovary (ascites)
Cell characteristics	epithelial, adherent
Tumorigenic in nude mice	Yes
Genetic background	SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin.
Karyotype	hypodiploid to hypotetraploid
Antigen expression	Blood Type B; Rh+
STR Profile	Amelogenin: X CSF1PO: 11 D13S317: 8,11 D16S539: 12 D5S818: 11 D7S820: 13,14 THO1: 9,9.3 TPOX: 8,11 vWA: 17,18
Virus
Parental cell line
Doubling Time
Culture Medium	McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs	SGI-110
EpiONC
EpiTSG

SNU-387

General Information
Cancer	pleomorphic hepatocellular carcinoma (grade IV/V)
Gender	female
Age	41 years
Tissue	liver
Cell characteristics	epithelial, adherent
Genetic background	Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Antigen expression	Blood Type O; Rh +
STR Profile	Amelogenin: X CSF1PO: 13,14 D13S317: 12 D16S539: 9 D5S818: 10,12 D7S820: 8,10 THO1: 7 TPOX: 8,9 vWA: 14,16
Virus	HBV
Doubling Time	60h
Experimental Data
EpiDrugs	SGI-110

SNU-398

General Information
Cancer	hepatocellular carcinoma
Gender	male
Age	42 years
Tissue	liver
Cell characteristics	epithelial, mixed adherent and floating
Genetic background	Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Karyotype	aneuploid; modal number = 62
Antigen expression	Blood Type O; Rh +
STR Profile	Amelogenin: X,Y CSF1PO: 13 D13S317: 11 D16S539: 10,14 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 11 vWA: 17,18
Virus	HBV
Doubling Time	39h
Experimental Data
EpiDrugs	SGI-110

SNU-449

General Information
Cancer	hepatocellular carcinoma (grade II-III/IV)
Gender	male
Age	52 years
Tissue	liver
Cell characteristics	epithelial (diffusely spreading cells), adherent
Genetic background	Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Karyotype	aneuploid; modal number = 57
Antigen expression	HBV
Doubling Time	36h
Experimental Data
EpiDrugs	SGI-110

SNU-475

General Information
Cancer	hepatocellular carcinoma (grade II-IV/V)
Gender	male
Age	43 years
Tissue	liver
Cell characteristics	epithelial, adherent and monolayer of diffusely spreading cells
Genetic background	Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Karyotype	aneuploid; modal number = 61
Antigen expression	Blood Type AB; Rh +
STR Profile	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 12 D5S818: 10,13 D7S820: 7,12 THO1: 7,9 TPOX: 8,9 vWA: 14
Virus	HBV
Doubling Time	66h
Experimental Data
EpiDrugs	SGI-110

T

TC-71