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Cancer Epigenetics Cell Line Database 2017-05-19T11:28:30+00:00

Welcome to the Cancer Epigenetics Cell Line Database (CECD)! Here you will find an ID card summarizing some of the known properties for each cell line reported in the Cancer Epigenetics Drug Database (CEDD). One may also access this page by clicking on the name of the cell line in the Cancer Epigenetics Drug Database.

This page is periodically updated, so please check it again for new data that may be relevant to your research. You are welcome to contribute to this database and may do so by emailing us the data and source of information at info@ces.b2sg.org, Subject line: CECD.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

22Rv1

General Information
Cancer Human prostate carcinoma
Gender Male
Tissue Prostate
Cell characteristics Epithelial, adherent
Tumorigenic in nude mice Yes
Genetic background Carries more than 10 genome-integrated copies of the xenotropic murine leukemia virus-related virus
p53 status Q331R
Karyotype
49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+1,+3,+7,+8,+12
Antigen expression Prostate-specific antigen (PSA)
STR Profile Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12,13
D7S820: 9,10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21
Virus EBV, HBV, HCV, HIV, HTLVI, HTLVII, SMRV, XMRV+
Parental cell line CWR22R
Doubling Time
40 hours
Culture Medium RPMI-1640
Additional Information Active viruses are secreted into the cell culture supernatant
Experimental Data
EpiDrugs Hydralazine

6-10B

General Information
Cancer Human nasopharyngeal cancer
Cell characteristics Low tumorigenicity, non-metastatic
Virus EBV+
Parental cell line SUNE-1
Culture Medium RPMI-1640 + 10% FBS
Experimental Data
EpiDrugs 5-aza-2′-deoxycytidine

A

A2780

General Information
Cancer Human ovarian carcinoma
Gender Female
Tissue Ovary
Cell characteristics Epithelial, adherent
p53 status
V172F
Other mutations
ARID1A, BRAF, PIK3CA, PTEN
STR Profile Amelogenin: X
CSF1PO: 10,11
D13S317: 12,13
D16S539: 11,13
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16
Culture Medium RPMI-1640 + 2mM glutamine + 10% FBS
Additional information Established from an untreated patient
Experimental Data
EpiDrugs SGI-110

A4573

General Information
Cancer Human Ewing’s sarcoma
Gender Female
Experimental Data
EpiDrugs 5-aza-2′-deoxycytidine

A549

General Information
Cancer Human lung carcinoma
Gender Male, causasian
Age 58 years
Tissue Lung
Cell characteristics Adherent, epithelial
Antigen expression Positive for keratin by immunoperoxidase staining
STR Profile Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14
Doubling Time About 22 hours
Culture Medium Ham′s F12K or DMEM + 2mM glutamine + 10% FBS
Additional Information Synthesizes lecithin
Experimental Data
EpiDrugs 5-aza-2′-deoxycytidine

A673

General Information
Cancer Human Ewing’s sarcoma
Gender Female
Age 15
Tissue Muscle
Cell characteristics Adherent
Tumorigenic in nude mice Yes
STR Profile Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18
Culture medium DMEM + 10% FBS
Experimental Data
EpiDrugs 5-aza-2′-deoxycytidine

C

CaSki

General Information
Cancer Human epidermoid carcinoma
Gender Female
Age 40 years
Tissue Cervix; derived from a metastatic site in the small intestine
Cell characteristics Epithelial, adherent
Genetic background
PIK3CA (heterozygous for E545K)
Antigen expression Tumor-associated antigen; human chorionic gonadotropin hCGβ
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17
Virus
HPV-16 (~600 copies/cell)
Experimental Data
EpiDrugs Hydralazine

CNE-1

General Information
Cancer Human nasopharyngeal cancer
Experimental Data
EpiDrugs 5-aza-2’-deoxycytidine

D

DU-145

General Information
Cancer Human prostate carcinoma
Gender Male
Age 69 years
Tissue
Prostate; derived from a metastatic site: brain
Cell characteristics Epithelial, adherent
Tumorigenic in nude mice
Yes, forms adenocarcinoma, grade II
Genetic background Hypotriploid (61 and 62 chromosome counts)
Karyotype
Antigen expression
Blood Type O; Rh+
STR Profile
Amelogenin: X, Y
D7S820: 7, 10, 11
D13S317: 12, 13, 14
D5S818: 10, 13
vWA: 17, 18, 19
THO1: 7
CSF1PO: 10, 11
TPOX: 11
D16S539: 11, 13
Additional information
Does not appear to be hormone-sensitive, is only weakly acid phosphatase positive, does not express prostate-specific antigen (PSA). Isolated cells form colonies in soft agar.
Experimental Data
EpiDrugs Genistein, Hydralazine

H

H1975

General Information
Cancer Non-small cell lung cancer, adenocarcinoma
Gender Female
Tissue Lung
Cell characteristics Epithelial, adherent
Genetic background Tumor is from a non-smoker
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10,13
D16S539: 9,12
D5S818: 11,12
D7S820: 8,11
THO1: 7
TPOX: 8,11
vWA: 18
Experimental Data
EpiDrugs 5-aza-2’-deoxycytidine

HCT116

General Information
Cancer Colorectal carcinoma
Gender Male
Age bracket Adult
Tissue
Colon
Cell characteristics Epithelial, adherent
Tumorigenic in nude mice Yes
Genetic background
Mutant Ras (codon 13)
Antigen expression
Carcinoembryonic antigen (CEA)
STR Profile
Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8,9
vWA: 17,22
Experimental Data
EpiDrugs 5-azacytidine

HeLa

General Information
Cancer
Cervical carcinoma
Gender Female
Age 31
Tissue Cervix
Cell characteristics Epithelial, adherent
Genetic background
Low p53 expression, normal pRB levels
STR Profile Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Virus
EBV, HBV, HCV, HHV-8, HIV, HPV-18+, HTLV-I/II, MLV, SMRV
Doubling Time 48h
Experimental Data
EpiDrugs 5-azacytidine, Hydralazine

Hep3B

General Information
Cancer Hepatocellular carcinoma
Gender Male
Age 8 years
Tissue Liver
Cell characteristics Epithelial, adherent
Tumorigenic in nude mice Yes
Genetic background
Contains a 2.3 kb integrated hepatitis B virus genome fragment
Antigen expression Hepatitis B surface antigen (HBsAg)
STR Profile
Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17
Virus EBV, HBV+, HCV, HIV, HTLV-I/II, MLV, SMRV
Doubling Time
40-50 hours
Culture Medium
90% MEM (with Earle’s salts) + 10% heat-inactivated FBS + 2 mM L-glutamine
Additional information
Produces α-fetoprotein, albumin, transferrin, α2-macroglobulin, α1-antitrypsin, and haptoglobin
Experimental Data
EpiDrugs SGI-110

Hep2G

General Information
Cancer
cervix carcinoma
Gender female
Age 31
Tissue cervix
Cell characteristics epithelial, adherent
Genetic background
HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.
P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
STR Profile Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Virus
EBV -, HBV -, HCV -, HHV-8 -, HIV -, HPV +, HTLV-I/II -, MLV -, SMRV –
Doubling Time 48h
Experimental Data
EpiDrugs 5-azacytidine Hydralazine

HLE

General Information
Cancer hepatoma (non-differentiated)
Gender 68 years
Age male
Tissue liver, gallbladder
Virus CMV-, EBV-, HHV6-, HHV7-, BKV-, JCV-, ADV-, parvoB19-, HBV-, HTLV1-, HTLV2-, HIV1-, HIV2-, HPV18-
Culture Medium Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum
Experimental Data
EpiDrugs 5-azacytidine

HLF

General Information
Cancer
cervix carcinoma
Gender female
Age 31
Tissue cervix
Cell characteristics epithelial, adherent
Genetic background
HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.
P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
STR Profile Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Virus
EBV -, HBV -, HCV -, HHV-8 -, HIV -, HPV +, HTLV-I/II -, MLV -, SMRV –
Doubling Time 48h
Experimental Data
EpiDrugs 5-azacytidine Hydralazine

Huh7

General Information
Cancer hepato cellular carcinoma
Gender male
Age 57 years
Tissue liver, gallbladder
Cell characteristics epithelial
Genetic background well differenciated hepatoma, infinite lifespan
Virus CMV-, EBV-, HHV6-, HHV7-,BKJ-, JCV-, ADV1-, parvoB19-, HBV-, HTLV1-, HTLV2-, HIV1-, HIV2-
Culture Medium Dulbecco’s minimal essential medium with 10% fetal bovine serum
Experimental Data
EpiDrugs 5-azacytidine

K

KG-1

General Information
Cancer erythroleukemia
Gender male
Age 59 years
Tissue bone marrow
Cell characteristics myeloblast-like
Genetic background

KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

STR Profile Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Culture Medium IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs 5-aza-2′-deoxycytidine

KYSE150

General Information
Cancer esophageal squamous cell carcinoma
Gender female
Age 49 years
Tissue
upper (cervical) esophagus
Cell characteristics epithelial, adherent
Tumorigenic in nude mice yes
Genetic background Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures)
Virus EBV -, HBV -, HCV -, HHV-8 -, HIV -, HPV -, HTLV-I/II -, MLV -, SMRV –
Doubling Time 20-25 h
Experimental Data
EpiDrugs 5-fluoro-2′-deoxycytidine

L

LNCaP

General Information
Cancer
prostate carcinoma
Gender male
Age 50 years
Tissue metastasis (left supraclavicular lymph node ), prostate
Cell characteristics epithelial, adherent (single cells and loosley attached clusters)
Tumorigenic in nude mice Yes
Genetic background
Antigen expression prostate specific antigen
STR Profile Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 10,12
D16S539: 11
D5S818: 11,12
D7S820: 9.1,10.3
THO1: 9
TPOX: 8,9
vWA: 16,18
Doubling Time 34h
Culture Medium 80% RPMI 1640 + 20% h.i. FBS
Experimental Data
EpiDrugs Hydralazine

M

MCF7

General Information
Cancer breast adenocarcinoma
Gender female
Age 69 years
Tissue metastatic site (mammary gland), pleural effusion
Cell characteristics epithelial, adherent
Genetic background The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.
Karyotype
Antigen expression Blood Type O; Rh+cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin –
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Viruses EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV –
50h
Culture Medium 90% RPMI 1640 + 10% h.i. FBS + MEM non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin
Experimental Data
EpiDrugs 5-fluoro-2′-deoxycytidine Genistein

MDA-MB-231

General Information
Cancer breast cancer
Gender female
Age 51 years
Tissue metastatic site (mammary gland/breast; pleural effusion)
Cell characteristics epitherlial, adherent
Tumorigenic in nude mice Yes (forms poorly differentiated adenocarcinoma)
Genetic background Modal no.′s 62 and 64, near triploid
Karyotype
STR Profile Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18
Culture Medium L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS
Experimental Data
EpiDrugs Genistein

MRC-5

General Information
Cancer non
Gender male
Age 14 weeks gestation
Tissue lung
Cell characteristics fibroblast, adherent
Tumorigenic in nude mice no
Genetic background he cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38
STR Profile Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15
Culture Medium EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs Genistein

P

PC3

General Information
Cancer prostate adenocarcinoma, grade IV
Gender male
Age 62 years
Tissue metastatic site (bone)
Cell characteristics epithelial
Tumorigenic in nude mice yes
Genetic background
The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
Antigen expression HLA A1, A9
STR Profile Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17
Culture Medium EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs Hydralazine Genistein

R

RPMI8226

General Information
Cancer plasmacytoma, myeloma
Gender male
Age 61 years
Tissue peripheral blood
Cell characteristics lymphoblast, suspension
Genetic background There is no evidence of heavy chain production (cytoplasmic or secreted).
Karyotype Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres.
Antigen expression HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
STR Profile Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
Culture Medium RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs 5-azacytidine

S

SiHa

General Information
Cancer squamous cell carcinoma (grade II)
Gender female
Age 55 years
Tissue cervix
Cell characteristics epithelial, adherent
Tumorigenic in nude mice Yes
Genetic background Oncogenes: p53 +; pRB +
STR Profile Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 12
D5S818: 9
D7S820: 10
THO1: 6,9
TPOX: 8
vWA: 14,17
Experimental Data
EpiDrugs 5-azacytidine; Hydralazine

SKOV3

General Information
Cancer adenocarcinoma
Gender female
Age 64 years
Tissue ovary (ascites)
Cell characteristics epithelial, adherent
Tumorigenic in nude mice Yes
Genetic background SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin.
Karyotype hypodiploid to hypotetraploid
Antigen expression
Blood Type B; Rh+
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18
Virus
Parental cell line
Doubling Time
Culture Medium McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)
Experimental Data
EpiDrugs SGI-110
EpiONC
EpiTSG

SNU-387

General Information
Cancer pleomorphic hepatocellular carcinoma (grade IV/V)
Gender female
Age 41 years
Tissue liver
Cell characteristics epithelial, adherent
Genetic background
Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Antigen expression Blood Type O; Rh +
STR Profile Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 9
D5S818: 10,12
D7S820: 8,10
THO1: 7
TPOX: 8,9
vWA: 14,16
Virus HBV
Doubling Time 60h
Experimental Data
EpiDrugs SGI-110

SNU-398

General Information
Cancer hepatocellular carcinoma
Gender male
Age 42 years
Tissue liver
Cell characteristics epithelial, mixed adherent and floating
Genetic background Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed.
Karyotype
aneuploid; modal number = 62
Antigen expression Blood Type O; Rh +
STR Profile Amelogenin: X,Y
CSF1PO: 13
D13S317: 11
D16S539: 10,14
D5S818: 12
D7S820: 10,11
THO1: 7,9
TPOX: 11
vWA: 17,18
Virus HBV
Doubling Time 39h
Experimental Data
EpiDrugs SGI-110

SNU-449

General Information
Cancer hepatocellular carcinoma (grade II-III/IV)
Gender male
Age 52 years
Tissue liver
Cell characteristics epithelial (diffusely spreading cells), adherent
Genetic background Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Karyotype aneuploid; modal number = 57
Antigen expression HBV
Doubling Time 36h
Experimental Data
EpiDrugs SGI-110

SNU-475

General Information
Cancer hepatocellular carcinoma (grade II-IV/V)
Gender male
Age 43 years
Tissue liver
Cell characteristics epithelial, adherent and monolayer of diffusely spreading cells
Genetic background Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed.
Karyotype aneuploid; modal number = 61
Antigen expression Blood Type AB; Rh +
STR Profile Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12
D5S818: 10,13
D7S820: 7,12
THO1: 7,9
TPOX: 8,9
vWA: 14
Virus HBV
Doubling Time 66h
Experimental Data
EpiDrugs SGI-110

T

TC-71