An LSRP position is available in the laboratory of Dr. Matt van de Rijn in the Department of Pathology at Stanford University.

Our lab applies cutting-edge high-throughput methods to study soft tissue sarcomas. We seek to understand the molecular underpinnings of these cancers, with the goal of developing new diagnostic, prognostic, and/or predictive biomarkers and therapeutic targets that can be applied in clinical practice.  Our research interests include the genetic, epigenetic, and transcriptomic characteristics of various types of soft tissue tumors including leiomyosarcoma and leiomyoma. The LSRP will be involved in a research project that aims to perform molecular analyses of these tumors on a single cell level, in order to better understand the biology, evolution and clinical behavior of these tumors, and to identify novel therapeutic strategies. Specifically, LSRP will be making single cell and single nuclei preparations from different types of soft tissue tumors, doing molecular profiling (DNA sequencing, RNA sequencing, and methylome profiling) and apply computational biology methods to analyze and interpret the next generation sequencing data.  The position also includes lab management tasks such as ordering supplies, maintaining our slide/block/tissue inventory, keeping detailed lab records, and adhering to safety regulations.

We welcome candidates with a growth mindset, interest in a variety of cancer research questions related to molecular underpinnings of disease, and a willingness to take on challenging learning opportunities.

In addition to comparable research compensation, candidates will have the opportunity to learn and to develop their C.V. or resume through research publications and presentations.

Duties include:

•  Plan and perform experiments in support of research projects in the laboratory based on knowledge of scientific theory. Interpret and perform basic analyses of the results.
• Apply protocols for preparation of single cell and/or single nuclei suspension from fresh, frozen, and/or formalin fixed paraffin embedded tissue and subsequent library construction for next generation sequencing (sequencing performed at a core laboratory).  Sequencing data generated can be analyzed using existing software programs.  Downstream experiments may include designing a targeted sequencing test, in situ hybridization, and/or immunohistochemical biomarker testing.
• Review literature on an ongoing basis to remain current with new procedures and related research.
• Apply the theories and methods of molecular and/or computational biology to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols under supervision of a senior researcher.
• Maintain detailed records of experiments and outcomes.
• Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
• Help with general lab maintenance as needed; maintain lab stocks, manage chemical inventory and safety records, and provide general lab support as needed.
• Assist with orientation and training of new staff or students on lab procedures or techniques.

DESIRED QUALIFICATIONS:

• A bachelor’s degree in biological sciences or a related field.
• Previous experience or established knowledge of basic laboratory techniques in molecular biology.
• Strong analytical skills and excellent judgment.
• Ability to work under deadlines with general guidance is essential.
• Must be accurate, efficient, organized and have excellent record keeping abilities.
• Ability to work with team members in a cooperative fashion.
• Excellent communication skills.

EDUCATION & EXPERIENCE (REQUIRED):

Bachelor’s degree in related scientific field.

KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):

• General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
• Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
• Ability to maintain detailed records of experiments and outcomes.
• General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
• Ability to work under deadlines with general guidance.
• Excellent organizational skills and demonstrated ability to accurately complete detailed work.